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Microbiology

Department of Microbiology

Introduction
For diagnostic support of various speciality and super speciality department in ESIC Medical College  Hospital, Bihta, the department of Microbiology was established in 2021 along with other diagnostic department like Biochemistry and Pathology. The department of Microbiology are equipped with state-of-the-art equipment’s. To operate these sophisticated instruments equipment’s the laboratories have a team of efficient, motivated and qualified doctors and technical staff who are consistently involved in producing effective results and upholding quality assurance. At present the department is having the following sections for its proper and smooth function.
1    Bacteriology
2    Mycobacteriology  
3    Clinical Parasitology 
4    Clinical Immunology
5    Virology  Molecular Lab

Academics
MBBS Course students are being taught by experienced and dedicated group of faculties. We are also in process to start DNB Course in the department of Microbiology. We are also expecting to start paramedical Course BMLT in the department.

Library
The department of Microbiology has more than 30 books of Microbiology and infection control in its department for Faculties  Students. 

Teaching and non-teaching staff
Sl no     Post     In position 
1.    Professor            1
2.    Associate Professor     1
3.    Assistant Professor    2
4.    Senior Resident     2
5.    Technical Staff     10

Department Diagnostic work 
Sl no    Bacteriology

1.Culture  Sensitivity     Urine     Blood 
        PUS    Sputum 
        Stool     Body fluid 
2.Direct Microscopy     Gram stain     Wet Mount 
        Acid Fast Stain    Special stain
        LPCB stain     KOH Mount for fungus

Sl no    Parasitology 
1.    Routine examination of stool      Ova and Cyst     
2.    Microfilariae          

Sl no    Mycobacteriology 
1.    Microscopic -Ziehl Nelson stain      Sputum     Body Fluid 
        Tissue     Urine 
2.    Gene Expert CBNAAT     Sputum     Body Fluid 

Sl no    Immunology  
1.    RA factor test      7.    ICT for NS1Ag, IgG  IgM
2.    CRP Test             8.    ICT for for HIV
3.    ASO Test            9.    ICT for for HCV
4.     Typhi Dot            10.    ICT for for HBsAG 
5.    RPR                   11.    Widal Test 
6.    ICT for PfPv Malaria Antigen.        

Sl no    Special Immunology ELISA
1.    ELISA For Dengue Ns1 Antigen 
2.    ELISA For Dengue IgM Antibodies 
3.    ELISA For HIV 
4.    ELISA For HBsAg 
5.    ELISA For HCV 

Sl no    Virology 
1.    RT-PCR test for COVID 19
2.    Rapid Antigen test for COVID 19

FACULTY DETAILS - DEPARTMENT OF MICROBIOLOGY

1 Prof. Dr. Md Khalid Rashid
MBBS, MD Microbiology,     
Professor  HOD

2 Prof Dr. Puranjay Saha
MBBS, MD Microbiology,     
Associate Professor

3 Dr. Swati Salila
MBBS, MD Microbiology,
Assistant Professor

4 Dr. Vidyut Prakash          
MBBS, MD Microbiology,    
Assistant Professor

5 Dr. Gaurav Singh
MBBS, MD Microbiology,
Senior Resident 

6 Dr. Sona
MBBS, MD Microbiology,
Senior Resident

Research  Publication -

Publication of Dr. Md Khalid Rashid, HOD  Professor
1. Shyamal Modi, Md Nazish Ayubi, Sanjeev Kumar, Md Khalid Rashid.  “Prevalence and antibiogram of Acinetobacter baumani complex isolated from various clinical specimen” .13th January 2024, International journal of Academic Medicine  Pharmacy, E-ISSN2687-5365, P-ISSN2753-6556
Original research article.
2. Shyamal Modi, Md Nazish Ayubi, Sanjeev Kumar, Md Khalid Rashid.   “Dynamics of Dengue fever infection across the seasons”. International journal of Medicine and public Health, Vol 14, Issue 1, Jan- March 2024. Original research article.
3. Prevalence and associated risk factor of human Intestinal Parasitic Infection in Rural Area, International Journal of Health Sciences ISSN 2550-6978, 6 St 47540-47548
4. A cross sectional anti sars cov-2 seroprevalence study among healthcare workers in a tertiary care hospital of eastern India, Journal of Dr NTR university of health sciences vol 11 issue 2 , April June 2022

Publication of Dr. Puranjay Saha, Associate Professor

1.Das S, Mallick A, Barik M, Sarkar S, Saha P, the emergence of clonally diverse carbapenem-resistant Enterobacter cloacae complex in west Bengal, India A dockyard of beta lactamase periling nosocomial infections, International Microbiology ,2023
2.Guha A, Hazra S, Rahman MA, Saha P, Biswas G, Sagar KG, SARS- CoV2 induced multisystem inflammatory syndrome in children MIS-C with seropositive scrub typhus. A case series, international journal of medical science and Diagnosis research,2023 Vol 7No.2 2023 March-April ,1-11 
3. Ghosh S, Banerjee S, Saha P, Sikder P.  Analysis of microbes and their sensitivity pattern in chronic otitis Media in west Bengal. Bengal Journal of otolaryngology and head neck surgery, Vol 28 No2. August 2020166-171
4. Kar C, Saha P, Chakrabarti N, Sarkar P. Histopathological study of post kala azar dermal leishmaniasis and its importance for diagnosis in a peripheral tertiary institution. Indian Journal of clinical and experimental Dermatology ,2019 Jan-March 51 9-15
5.Manadal A, Mondal t, Saha P, Kundu P, Antibiotics susceptibility pattern of bacterial strains isolated from children with upper respiratory tract infection . IOSR Journal of dental sciences, 2018 volume 17, Issue 11 ver.10 November 2018 ,PP 01-06
6.Saha P, Mandal AK, Mondal T, Kundu PK. Prevalence of positive widal test in comparison with clinical request in a tertiary care teaching hospital , West Bengal .ISOR  Journal of Dental and Medical Science ,2018 . Volume 17, Issue 11 ver.4 November 2018,PP
7. Mondal T, Mandal A, Saha P, Kundu P. Susceptibility pattern of vulvovaginal candida isolates against antifungal drugs in a tertiary care teaching hospital , West Bengal , India . IOSR Journal of Dental and Medical Sciences ,2018 89
8. Saha P, Mandal AK, Mondal T, Kundu PK. Evaluation of ASO Test and correction with clinical request in a tertiary care teaching hospital. An insight study. IOSR Journal of   Dental and Medical Sciences ,2018 17170
9. Saha P, Mondal AK, Mondal T, Kundu PK. Evaluation of ASO test and correlation with clinical request in a tertiary care teaching hospital. An insight study. IOSR Journal of Dental and Medical sciences,2018 Volume 17, Issue 9 Ver 2 September 2018 Pp 55-58
10. Saha P, Mondal T, Mandal AK, Kundu PK. Prevalence of vulvovaginal candidiasis and trichomoniasis among women of reproductive age in a rural tertiary care hospital in west Bengal, India. IOSR Journal of   Dental and Medical Sciences ,2018 Volume 17 Issue 9 ver 1 September 2018 PP 45-50
11. Saha P, Saha R. Diagnosis of MDR Tuberculosis at a tertiary care centre with CBNAAT technology – a two-year study. Journal of Evidence Based Medicine and Healthcare n, Vol 553 2018.
12. Roy Choudhary C, Saha P, Das PK, Sarkar S, Kuila SP. A Study of gall stone disease with special reference to H pylori infection in a tertiary care centre in India. Scholars Journal of Applied Medical Science, Aug 2018 68
13. Mandal AK, Mondal T, Saha P, Roy A, Kundu PK. An Insight investigation of Dengue in a Tertiary care Teaching Hospital, West Bengal. Journal of Pure and applied Microbiology ,2017 Sep 1 
14. Sarkar S, Roy H, Saha P, Sengupta M, Sarder K, Sengupta M. Cystic echinococcosis. A neglected disease at usual and unusual locations. Tropical Parasitology, 2017 Jan71 51
15. Singh RM, Sarkar S, Saha P, Nag S. Carbapenemase producing Enterobacteriaceae among urinary isolates scenario from a tertiary care hospital in eastern India. Journal of evaluation of Dental and Medical Science ,2014 Feb 1036 123-24
16. Sarkar S, Gupta MS, Saha P, Nag S. Comparison of in vitro activity of 0 popular fluroquinolones against common respiratory and middle year pathogens. National Journal of Otorhinolaryngology and Head and Neck Surgery ,2013 151952-78
17.Saha M, Yay S, Goswami M, Kundu S , Saha P , Saha A , Maitra S, Talukdar A. An occult filarial infection presenting as chyluria with proteinuria A case report and review of literature. BMJ Case Reports ,2012 bcr0120125635
19. Sarkar S, Saha P, Sengupta M. Cutaneous Nocardia brasiliensis infection in an immunocompetent host after ovarian cystectomy. A case study. Australasian Medical Journal, 2011 Nov 1411 603

PUBLICATIONS OF DR. VIDYUT PRAKASH

1. OVERVIEW OF INTESTINAL COCCIDIAN PARASITES IN HUMAN BEINGS. Microbes of Medical Importance e-ISBN  978-93-6252-020-3 IIP Series. Book Chapter by Dr Vidyut Prakash, Assistant Professor, ESIC MCH, Bihta
2. Prakash V, Saurabh K, Kumar R, Kumar R, Kumari N, Kumar S. Performance Evaluation of Rapid Antigen Test for COVID-19 with SARS-CoV-2 RT-PCR using Nasopharyngeal Swab with Special Reference to RNA Dependent RNA Polymerase RdRp Cycle Threshold Values. J Pure Appl Microbiol. Published online 20 November 2023. doi10.22207JPAM.17.4.28
3.  Prakash V, Singh RK, Saurabh K, et al. Spectrum of chemo-radiotherapy induced fungal infection in head and neck cancer patients at tertiary care centre of Eastern India. Oral Oncology Reports. 2023 6100039. Cross ref
4. Parwez A, Singh S, Kumar R, Kumari R, Kumar V, Prakash V, Ali M. Determination and evaluation of HR-HPV genotype in different communities of Bihar, India. Int J Health Sci Qassim. 2022 Sep-Oct16540-48. PMID36101850 PMCID PMC9441651.
5. Raut AK, Ayubi MD, Kaushik C, Prakash V. Prevalence of Candida Species in Urinary Tract Infections in a Tertiary Care Hospital, Bihar. Journal of Cardiovascular Disease Research. VOL13, ISSUE 02, 2022
6. Prasad N, Prasad D, Prakash V, Singh K, Shashi K, Pankaj S, Bimal K. Seroprevalence of Rubella virus infection in susceptible women of childbearing age group seeking preconceptional counselling and infertility treatment a Cross-Sectional study from Eastern India. National Journal of Laboratory Medicine. 2022
7. Prakash V, Prasad N, Shahi SK. Trend of HIV Infection and Associated Risk Factors at Integrated Counselling and Testing Centre in a Tertiary Care Hospital in Bihar A Retrospective Study. National Journal of Laboratory Medicine. 2021 Apr, Vol-102 PO01-PO04
8. Prakash V, Prasad N, Prasad D, Shahi SK. Hepatitis B Virus Seroprevalence amongst Pregnant Women Attending Antenatal Clinics of a Tertiary Care Centre of Bihar. Sch J App Med Sci, Feb., 2020 82 409-412
9. Ayubi N, Prasad N, Prakash V, Bimal KK. Epidemiological Study of Dengue Fever at a Tertiary Care Centre in Patna.Sch J App Med Sci, Feb., 2020 82 749-752
10. Prasad D, Prasad N, Prakash V, Parven S, Mishra U. Sero-prevalence of unvaccinated adolescent girls susceptible to rubella virus infection seen in a tertiary care hospital of Patna, Bihar, India. Int J Repro’d Contracept Obstet Gynecol 2020 91972-5.
11. Chaubey L, Kumar D, Prakash V, Nath G. Menstrual blood versus endometrial biopsy in detection of genital tuberculosis by using nested polymerase chain reaction in an endemic region. J Hum Repro’d Sci 20191235-9.
12. Ahmad W, Prakash V, Kumari N  Jha AK. Bacteriological Profile and their Antibiotics Sensitivity of Ascitic Fluid in Patients of Cirrhosis in a Tertiary Care Hospital Patna, Bihar. International Journal of Medical Sciences and Innovative Research IJMSIR, 2019, Volume – 4, Issue – 1, February -, Page No. 12 – 17
13. Jha AK, Jha P, Chaudhary M, Kumar S, Prakash V, Dayal VM. The relation of toddy palm wine intake to amoebic liver abscess in a predominantly toddy-drinking population a cross-sectional study. Tropical Doctor, 2019, 00 1–4
14. Kumar S, Prakash V, Kumar R, Raut AK, Rajpal K, Singh R, Shahi SK. Utilization of Blood and its components in tertiary care Hospital of Eastern India. Asian Pac. J. Health Sci., 2019 6229-35
15. Muni S, Kumar R, Kumar S, Prakash V, Kumari N, Shahi SK. Current Trends of Various Candida Coinfection using KB006Hi Candida Kit and CHROM Agar in Pulmonary Tuberculosis Patients at a Tertiary Health Care Centre in Patna. Int J Sci Stud 2019731-4.
16. Ahmad W, Prakash V, Kumar R, Kumar S, Xess A. Prevalence of Intestinal Parasitic Infection in Patients Attending Tertiary Care Hospitals of Patna, Bihar. Sch. J. App. Med. Sci., Dec, 2018 612 4977-4981
17. Prasad N, Prakash V, Bimal K K, Shahi, S.K. Seroprevalence of Dengue Infection in Patients Suffering from Acute Febrile Illness in a Tertiary Care Centre–Time to Introduce Dengue Vaccine CYD-TDV to Susceptible Population. Int. J. Curr. Microbiol.App.Sci. 2018 7061079-1085.
18. Samanta S, Prakash V, Nayak J, Kumar D. Prevalence of High and Low -level Mupirocin, resistance against MRSA from a tertiary care hospital in eastern UP. Asian Pac. J. Health Sci., 2018 53212- 215
19. Prasad N, Prakash V, Sharma R, Kumar S, Shahi S K. Seroprevalence of HBV, HCV and HIV infection among blood donors at a tertiary care centre in Eastern India-Time to introduce molecular methods for follow up management of all seropositive donors. International Journal of Medical Sciences and Innovative Research IJMSIR , 2018 Volume – 3, Issue –3, June -, Page No. 80 – 85
20. Prakash V, Kumar N, Kumari Namrata, Singh Neelima, PriyaPallavi, Shahi SK. Permanent Pacemaker Infection by Pasteurella aerogenes- a rare case report from Bihar. JIGIMS, 2018, Vol. 4, No. 2
21. Prakash V, Kumar D, Singh S, Nath G. Bacterial profiling of healthy bone marrow using polymerase chain reaction Technique. Iraqi J Hematol 2017 669-73
22. Ravi T, Deepak K, Nayak , Kumar M, Prakash V. In-Vitro Activity of Teicoplanin against Clinical Methicillin Resistant Staphylococcus aureus Isolates. J Microbiol Biotechnol, 2017, 21 000112.
23. Kumar D, Prakash V, Nat h G. Prevalence of polymorphisms at position 86 of the Pfmdr1 gene in Plasmodium falciparum parasites. Parasitol United J 2016 9103-105
24. Kumar D, Kaushal S K, Kumar G, Prakash V et al. Evaluation of the antibacterial activity of commonly used alcohol based hand sanitizer on common pathogenic bacteria. Indian journal of applied research, Volume 5  Issue 3  March 2015
25. Muni Sweta, Prakash V, Kumar R, Kumar S, Pankaj Deepak. Primary Breast Tuberculosis Presenting as a Lump A refractory case from Eastern India. JIGIMS

PUBLICATIONS OF DR. SWATI SALILA

1.Priyamvada, Salila S, Blood donor deferral analysis in relation to the screening process in blood centre, ESIMCH,
Bihta, Patna.
2. Keche A, Nagarkar NM, Salila S, Arora RD, Mehta R, Tigga R. Multiple amp Appropriate Sampling Proved as Key for Early Diagnosis of CAM. Acta Scientific Otolaryngology 2022 Jan422-7 CORRESPONDING AUTHOR
3. Salila S, Gaikwad UN. Diagnostic Value of Post Decontamination Smear Microscopy for Enhanced Detection of Tuberculosis. Int J of Current Res amp Review 2022 Feb 140487-90
4. Salila S, Kumar R, Kumar S, Kumari N, Xess A. Tuberculous Splenic Abscess in an Immunocompetent Child A case Report. Ind J of Applied Research 2021 Nov
5. Xess A, Salila S. Blastoschizomyces capitatus Pneumonia, mimicking Pulmonary Tuberculosis– a case report Int J of Contemporary Med Research 2020 Sep79. CORRESPONDING AUTHOR
6. Salila S, Kumar R, Kumar S, Kumari N, Xess A, Shahi S K. Isolated Tuberculous Liver Abscess In An Immunocompetent Adult Female Journal of IGIMS 2019 Aug 52
7. Salila S. Distribution and Characterization of Candida Species in Various Clinical Samples at a Tertiary Care Hospital. Ind J of Applied Research 2018 Feb 82
8. Kumar R, Kumar R, Shahi SK, Salila S, Kumar S, Mishra B. Early diagnosis of smear negative pulmonary tuberculosis a two-year study from tertiary care centre. International Journal of Contemporary Medical Research 2018 52B1-B4.
9. Salila S, Kumar S, Vikram K, Kumar R, Kumar S, Xess A, Shahi S K. Sero-Prevalence and Co-Infection of Hepatitis A amp E Virus In Patients with Acute Viral Hepatitis at A Tertiary Care Hospital. Int J of Cont. Med 2017 Jan-Jun 5195-98.
10. Kumar R, Kumar S, Salila S, Vikram K, Kumari N, Xess A, Shahi S K. Prevalence of HIV among ICTC Attendees at Indira Gandhi Institute of Medical Sciences, Patna, Bihar. Ind J of Applied Research 2017 Apr 74183-185. CORRESPONDING AUTHOR
11. Kumar R, Vikram K, Salila S, Kumar S, Kumari N, Xess A, Shahi SK. Rapid Detection of Mycobacterium tuberculosis and Rifampicin resistance in extra-pulmonary samples using GeneXpert MTBRIF assay. J of Med Sci and Clin Res 2017 5139-41. CORRESPONDING AUTHOR
12. Kumar S, Salila S, Kumar R, Kumar S, Xess A, Shahi S K. HIV, HCV amp HBV Seroprevalence among Blood Donors in a Tertiary Care Hospital  A five year Study. Int J of Cont Med 2017 Jul-Aug 93143-146.
13. Muni S, Kumar A, Shahi SK, Pankaj D, Sen A, Salila S. Diarrhoea with Special Reference to Enteropathogenic E.coli in Children below Five Years at a Tertiary Care Centre. Int J of Contemporary Med Research 2017 Aug-Sep491934-37.
14. Kumar S, Gupta KK, Kumar R, Salila S, Shahi SK. Localization of Staphylococcus aureus in Tissue from the Nasal Vestibule in Healthy Carriers. Ind Med J 2017 Jun 1116103-106A.
15. Kumar S, Gupta KK, Kumar R, Salila S, Shahi SK. Dominant Bacterial Phyla in Caves and their Predicted Functional Roles in C and N Cycle. Ind Med J 2017 Jun 111686-90A.
16. Kumar S, Kumar S, Ranjan R, Salila S, Kumar M, Kumari N, Shankar M, Shahi SK. Distribution of Biofilm Producing Di 1 erent Candida Species from Various Clinical Samples at a Tertiary Health Centre in Bihar. National J of Integ Res in Med 2016 Nov-Dec 76106-109.

Awards  Recognition

Prof Dr. Khalid Rashid, M.D Microbiology 

Workshops -10
Conference  CME – 10
  
Dr. Swati Salila , M.D  Microbiology 

Workshops - International -02 , National -08
Conference  CME – International -2 , National -07, State - 11
  
Dr. Vidyut Prakash  , M.D  Microbiology 

Workshops - National -05
Conference  CME –National -07 , State- 06
Certificate Course in Critical Care Infectious Disease Course CCIDC
Certificate Course in Evidence Based Diabetes Management CCEBDM

Last updated / Reviewed : 2025-01-03